Production Optimization and Industrial Applications of Amylase From Indigenous Bacterial Species Using Banana Peels

Introduction: Alpha amylases are starch hydrolyzing enzymes that possess high industrial demand. Various strategies have been adopted to enhance cost-effective enzyme production of which utilization agro-industrial waste is very promising. Methodology: In this study two amylase producers Bacillus megaterium and Exiguobacterium auranticum were isolated identified by 16sRNA sequencing their growth conditions optimized via submerged fermentation using banana peels as carbon source. found be mesophilic alkaline (37℃, pH 9) strain giving 472 U/ml while acidic thermophilic (50°C, 5) gave 391 U/ml. Results Conclusion: Production E. reported here for the first time. The crude also showed better chocolate curry stain removal capacity when combined with commercial detergent. Also juice clarification assay promising results indicates could potentially used in detergent food industry.